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Juliane Liepe (Imperial College London, London, United Kingdom)We investigate leukocyte migration processes in zebrafish embryos during inflammation, which as part of the innate immune system form the first line of response to infection and trauma. Fluorescence microscopy has been used to generate time-lapse images that follow migrating leukocytes during inflammation. Image processing, including object detection and tracking, allows statistical analysis of the leucocyte trajectories as a function of time and space. With this information we can link intracellular signalling dynamics to tissue-level processes. Immune response mechanisms span both scales and automated image analysis will allow us to elucidate this intrinsically multi-scale problem. Zebrafish embryos, because of their transparency, provide a powerful model organism to study the spatio-temporal aspects of the innate immune response in vivo; in particular for the inference of mathematical multi-scale models it has probably unique advantages. We will illustrate the power of the model system in describing multi-scale dynamics by coupling image processing with explicit mathematical models for leukocyte chemotaxis.