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Maria Secrier (EMBL, Heidelberg, Germany)Biological systems are complex, dynamic entities, the behavior of which is difficult to capture in a holistic manner over space and time. Visualization tools can greatly enhance the ability to explore the properties of such systems by exploiting man's innate capacity to visually perceive hidden features in the represented data. Several tools for the visualization of biological networks exist, but integrating the spatial and temporal information for a specific system remains a major bottleneck in systems biology. The Arena3D software enables the users to investigate a wide variety of networks and connections between systems at different levels, as well as their evolution in time, by emphasizing changes in gene expression and highlighting pathways that are activated at different stages. The 3D framework allows the user to easily understand the connections that appear between different biological levels. The ability to depict both static connections and dynamic changes in a variety of datasets is illustrated. Providing an enriched informational content through spatio-temporal integration has the potential of enabling the discovery of new relationships between biomolecules, links betwe