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Franck Debarbieux (IBDML, Marseille, France)A challenging issue in central nervous system pathologies is to describe the dynamic interplay taking place between cells from the nervous, vascular and immune systems and to identify their effects. Most of the imaging and transgenic animal tools are now available to address these questions. We have developed a fluorescence imaging protocol and its associated mouse models of pathologies to study how the vascular system contributes to axonal regeneration following spinal cord injury (SCI) and to tumor development in glioblastoma. (GBM). For SCI, high-resolution images showed that neurovascular interactions occurred within 2 weeks post injury. Timelapse analysis showed that neovessels exerted a potent growth stimulating action, but no guidance effect on neighboring sprouts. For GBM usually described as highly vascularized, we found that at least part of tumor vessels resulted from remodeling of the local brain vascularization. Plastic changes were associated with local disruption of brain blood barrier as evidenced by the leak of fluorescent low molecular weight dextrans injected intraveinously. We now evaluate the opportunity to manipulate angiogenesis as therapeutic strategies.