Getting poster data...
Jan Huisken (Max Planck Institute of Molecular Cell Biology)We observed that our implementations of SPIM have enabled time-lapse recordings of superior speed and minimal phototoxicity. Experiments have become possible that run at full speed using the best possible hardware, without being limited by the fragility of the sample. High-speed 3D data can be recorded non-stop. The speed advantage of the SPIM over other fluorescence tech-nique can be utilized to image rapid events in developing tissue or to record a large number of views for multi-view reconstruction. The large amount of data that is accumulated when modern cameras are run at high-speed for hours or days is enormous and innovative data processing solu-tions are needed. Therefore, the challenges in microscopy are moving now from sample prepara-tion and preservation to data storage, analysis and most importantly visualization. Examples of our work include the multi-dimensional reconstruction of the zebrafish vasculature during devel-opment over several days, the analysis of cardiac development, function and conduction system as well as the tracking of endodermal cells on spherical surfaces.