Getting poster data...
Alain Meil and Patrick Durand (Korilog - 4, rue Gustave Eiffel - 56230 Questembert - France - email@example.com)Functional sequence analysis usually requires a homology search against reference annotated databases. When working with NGS data sets, the key issue is to provide the biologist with a tool capable of facilitating the management and the deciphering of large result sets. We propose a new tool, the GlobalViewer, which is part of the sequence analysis platform KoriBlast, to target the functional analysis of NGS sequence datasets. Its main feature lies is its capability to display a global interactive view of the results produced by Blast comparisons as a 2D dotplot. This representation mode offers the possibility to assess globally a medium size result dataset (about 1 million sequences) in a first approach and then to interactively explore some areas of interest taking advantage of the biological classifications associated to the hits. We present a use case 2: an environmental comparative metagenomics project in which the tool was used to examine the consequences of C/N ratio modifications on denitrification process. The study is based on the enzymatic annotations obtained after Blast comparisons of sequences extracted from groundwater samples.