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David E. Breen, Thomas Widmann, Linge Bai, Liyuan Sui, Frank Jülicher, Christian Dahmann (Drexel University, Philadelphia, PA, USA; GENYO, Granada, Spain; MPI-PKS, Dresden, Germany; TU Dresden, Germany)The size and shape of biological tissues depend on the number, size, shape, and arrangement of the constituting cells. To better understand the mechanisms that guide tissues into their final shape, it is important to investigate the cellular arrangement within tissues. In this poster we present a set of techniques that generates 3D volumetric models of epithelial tissues, as well as geometric descriptions of the tissues’ individual cells. The input to the reconstruction process is a stack of confocal microscopy images. Once the reconstruction is completed, geometric calculations are performed to compute the apical cross-sectional areas, heights and volumes of the individual cells. Using these quantities color-based visualization of a tissue's morphological parameters is performed. In their first utilization we have applied these techniques to construct 3D volumetric models at cellular resolution of the wing imaginal disc of Drosophila melanogaster. The calculation and visualization of morphological parameters show position-dependent patterns of cell shape in the wing imaginal disc, and exhibit trends in cell parameters during wing disc development.