Getting poster data...
Heng-Chang Chen, Arbert Jordan, Guillaume Filion (Centre for Genomic Regulation (CRG), Edif. PRBB, Dr. Aiguader, 88, 08003, Barcelona, Spain )Human immunodeficiency virus type 1 (HIV-1) is the etiologic agent of the acquired immunodeficiency syndrome (AIDS), which is responsible for a pandemic affecting 34 million people worldwide. During the course of infection, HIV-1 integrates in the genome of the host. At that stage, a fraction of the viruses enter a silent infection mode called latency. The transcription of the virus shuts down, which makes it invisible to the immune system. Nowadays, latency is the main roadblock to the development of a cure for HIV-1. Accumulating evidence has indicated that chromatin context shows strong influence on determination of HIV-1 DNA integration in the host genome. However, there is no efficient technique available that allows us to detect where lante HIV-1 proviruses exact integration points are still. In this study, we therefore aim to develop a high-throughput technology named Single Integration Navigation (SIN) that benefits us to map every HIV-1 provirus integration in a cell population and measure its individual transcription in the same experiment. By exposing the integrations that produce no transcript, such dual maps will reveal the latent HIV-1 integrations.