Getting poster data...
Jelena Chuklina, Maxim Imakaev, Elena Evguenieva-Hackenberg, Mikhail Gelfand (Moscow Institute of Physics and Technology, Moscow, Russa; Institute for Information Transmission Problems, Moscow, Russia; MIT, Boston, USA)Here we present the transcriptome analysis based on dRNA-seq data of nitrogen fixing α-protebacterium, soy symbiont Bradyrhizobium japonicum USDA 110 with RNA taken from soy root nodules and free-living state. dRNA-seq allows one to map transcription start sites (TSSes) in bacteria, using terminal exonuclease (TEX) to degrade transcripts with processed 5’-ends. TSS map allows to reveal regulatory networks, the key players being transcription factors and small non-coding RNAs. The special type of transcription factors is sigma-factor of RNA-polymerase, which recognizes promoters thus launching transcription. As the promoter possesses both conserved sequence and position relative to TSS, it is possible to detect promoters de novo. We hypothesise that different sigma factors (in our case house-keeping sigma-70 or RpoD and nitrogen metabolism associated sigma-54 or RpoN) may underlie differential expression of protein-coding and sRNA coding genes during symbiosis or free-living state.