Getting poster data...
E. Wait, M. Winter, C. Bjornsson, S. Goderie, S. Temple, and A. Cohen (Drexel University, Philadelphia, PA; Neural Stem Cell Institute, Rensselaer, NY)The subventricular zone (SVZ) is one of the few places in the adult brain where neural stem cells are found. Here we see an intact SVZ (top) constructed from a montage of 82 overlapping 3-D image stacks. Each image stack is 1024x1024x80 voxels and contains five channels, labelling cell nuclei (blue), blood vessels (red), astrocytes and neural stem cells (green), oligodendrocytes (yellow) and migrating neuroblasts (cyan). The bottom right shows a portion of the top image at native (unscaled) resolution, with stem cells clustering around the blood vessel. On the bottom left we see a portion of a frame from a time lapse image sequence showing two daughter cells undergoing mitosis. Segmentation and tracking results are drawn over the image data along with the division plane showing the orientation of the daughter cells relative to the nearby blood vessel. These images were rendered interactively in our analysis tool at hardware frame rates with active shutter stereo visualization using NVIDIA GTX hardware.