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Eric van der Helm, Henrik Marcus Geertz-Hansen Hans Jasper Genee, Sailesh Malla, and Morten O. A. Sommer (Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark)With the advent of functional genomics, a major chal-lenge is the analysis and interpretation of the ever-increasing se-quencing data. deFUME is an easy-to-use web-based interface for processing and annotation of functional metagenomics sequencing data, tailored to meet the requirements of non-bioinformaticians. The web-server integrates multiple analysis steps into one single work-flow: read assembly, open reading frame prediction and finally anno-tation with BLAST, InterPro and GO classifiers. This provides a fast track from raw sequence to a comprehensive visual data overview that facilitates effortless inspection of gene function, clustering and distribution.