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Khan, M. J, Figueiredo, L.T., Aquino.V.H (University of Sao Paulo, Ribeirao Preto , Sao Paulo, Brazil)Human activities are responsible for the global environmental changes, resulting in an increasing number of vector- and rodent-borne diseases worldwide. Rodents and arthropods-borne viruses are important globally as emerging and re-emerging viruses and most of them are RNA viruses. Efficient and early diagnosis of these infections is very important to prevent their spread, to improve clinical management of the patients, as well as to protect livestock and domestic animals. Currently, available diagnostic methods such as immunoassays, polymerase chain reaction and virus isolation can detect only one or few viruses in a single assay. The DNA microarray platform has emerged as a diagnostic tool suitable for high throughput screening of pathogenic agents. The aim of this study was to develop a DNA microarray platform (RoboArboVirusChip) for detecting rodent- and arthropod-borne viruses, which belong to seven families. We found 412 viral species, tentative or established strains whose sequences are available in the GenBank. We obtained 2144 viral sequences from viral families mentioned. For selection of specific probes, the relevant open reading frame of viral sequences from multiple ge