Getting poster data...
Satoshi Nishimura ( Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan, and 2 Department of Cardiovascular Medicine, the University of Tokyo, Tokyo, Japan)We developed new soft- and hardware platform for in vivo visualization, We used two photon technique for, single cell morphology and behavior can be visualized in living animals. X- (resonance), Y-(galvano), and Z-(piezo) axis scanning was performed, and we also developed visualization software platform. We also developed new two photon, light-sheet microscope and minimized implantable one for living animals, and visualized living mice without anesthesia, by projection imaging systems. For post-processing, we used PIV methods for evaluation of physical factors. Using this system, we visualized the cell dynamics during thrombus development, and assessed dynamic cellular interplay to elucidate the underlying molecular mechanisms of cardiovascular events. Thrombus formation was triggered by ROS production by photochemical reactions or endothelial cell, and we also observed vascular smooth muscle cell reactions in arter. In conclusions, three dimensional and multi-modal analysis of living mice revealed the diseased conditions in single cell, and specific molecular levels.