Getting poster data...
Arnim Jenett, Pierre Affaticati, Elodie de Job, Laurie Riviere, Barbara Rizzi, Johanna Djian-Zaouche, Jean-Stephane Joly (Tefor Core Facility, Paris-Saclay Institute of Neuroscience, CNRS, Université Paris-Saclay, 91190 Gif-sur-Yvette, France)Communicating our findings (3D) in print (2D) is always a challenge. We therefore started to experiment with collages of multiple views from different angles of the same specimen, arranging in patterns, which help the recipient to perceive the overall 3D-structure of the depicted object. Here we demonstrate a couple of these approaches and their underlying data to elicit a discussion on how to solve the problem of 2D-representation of 3D-data. Collage of a rotation around two cardinal axis of a 5-day-old (5dpf) zebrafish larvae. This specimen expresses green fluorescent protein (GFP) in a subpopulation of putative visceral neurons. These neurons may be involved in the regulation of respiration and digestion, but this hypothesis still needs further confirmation. The expression pattern is visualized by direct volume rendering in green, it’s weakest parts were rendered in transparent blue and for the display of the surrounding structures. For easier perception we highlighted putative sensory groups of neurons by other colors (yellow, oral gustation; magenta/cyan, buccal gustation; red, gill innervation; orange, optomotoric?) The white matter (rich in cell membranes)